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Download vlc player for pc free windows 7High-resolution melting analysis for detection of mycobacterial DNA in cerebrospinal fluid. In recent years, the use of cerebrospinal fluid (CSF) for the diagnosis of tuberculous meningitis (TBM) has gained popularity. In South Africa, the delay in diagnosis of TB meningitis is contributing to a high mortality and morbidity. Culture-based diagnostics are time-consuming and their result is available only after 21 days. An early diagnosis can be improved by early initiation of antituberculous treatment. Thus, molecular-based methods to detect TBM-specific pathogens in CSF are of great importance. High-resolution melting analysis (HRMA) is an attractive alternative to conventional PCR methods. The study was aimed to test and validate the sensitivity and specificity of HRMA to detect TB DNA in CSF. In this pilot study, DNA from Mycobacterium tuberculosis was amplified by real-time PCR and mixed with DNA from an unrelated organism. A qualitative (no. of positive samples) and a quantitative (cycle threshold [Ct]) analysis were performed and compared with the conventional PCR results. The melting temperatures of the amplicons were determined and analyzed by the delta-Ct method. Of 17 CSF samples investigated, conventional PCR detected TBM DNA in 12 CSF samples but only in 6 samples did HRMA detect TBM DNA. Five other mycobacterial DNA targets were present in the CSF samples by both PCR methods; 4 were detected by HRMA, and the 5th one was detected by conventional PCR. A difference in Ct between the target DNA and the mixed DNA was observed for each amplicon, thereby emphasizing the benefit of this novel test to resolve mixed target DNA. HRMA is a promising method for the detection of mycobacterial DNA in CSF. Further investigation is required to determine the sensitivity and specificity of HRMA for detection of TBM-specific pathogens in CSF.Q: LinearLayout background and fill parent I've noticed a weird behavior when setting the background of a LinearLayout as a drawable. It has no effect if you just set the parent of the Layout to "fill_parent". Here's my code, that does not work if i set parent to "fill_parent" on the LinearLayout:




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